Enhanced Stem Nematode Resistance of Transgenic Sweetpotato Plants Expressing Oryzacystatin-I Gene

• 作 者: GAO Shang YU Bo ZHAI Hong HE S
• 机 构: Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture/Beijing Key Laborat
• 出 处: 中国农业科学（英文版）
• 发表日期: 2011年4期
• 关键词: Agrobacterium tumefaciens Ipomoea batatas (L.) Lain. Oryzacystatin-I gene stem n
• 基金项目: the Scientific Fund to Graduate Research and Innovation Projects of China Agricultural University;the Earmarked Fund for Modern Agro-Industry Technology Research System (Sweetpotato),China;国家自然科学基金;th

Enhanced stem nematode resistance of transgenic sweetpotato (cv. Lizixiang) was achieved using Oryzacyslatin-I (OCI)gene with Agrobacterium tumefaciens-mediated transformation. A. Tumefaciens strain EHA 105 harbors a binary vector pCAMBIA1301 with OCI gene, gusA gene and hptⅡ gene. Selection culture was conducted using 25 mg L-1 hygromycin. A total of 1715 plants were produced from the inoculated 1 450 cell aggregates of Lizixiang via somatic embryogenesis. GUS assay and PCR analysis of the putative transgenic plants randomly sampled showed that 90.54% of them were transgenic plants. Transgenic plants exhibited significantly enhanced resistance to stem nematodes compared to the untransformed control plants by the field evaluation with stem nematodes. Stable integration of the OCI gene into the genome of resistant transgenic plants was confirmed by Southern blot analysis, and the copy number of integrated OCI gene ranged from 1 to 4. Transgene overexpression in stem nematode-resistant plants was demonstrated by quantitative real-time PCR analysis. This study provides a way for improving stem nematode resistance in sweetpotato.