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目的探讨免疫抑制分子人类白细胞抗原G(HLA-G)在卵巢恶性肿瘤组织中的表达及其临床意义。方法采用免疫组织化学SP法,应用抗HLA-G单克隆抗体4H84,检测45例原发性卵巢恶性肿瘤和36例卵巢良性肿瘤组织中HLA-G蛋白的表达。应用逆转录酶聚合酶链式反应(RT-PCR)法分析新鲜卵巢良、恶性组织中HLA-G mR-NA的表达。结果①免疫组织化学显示,30例卵巢恶性肿瘤组织中有HLA-G的表达阳性(66.67%),HLA-G在卵巢恶性肿瘤组织中的表达明显高于良性组织(P<0.01);②浆液性囊腺癌、粘液性囊腺癌、子宫内膜样癌间HLA-G分子的阳性率差异有统计学意义(P<0.05),HLA-G阳性率与年龄、临床分期、病理分级、淋巴结转移无关;③RT-PCR结果表明卵巢恶性肿瘤组织中有HLA-G mRNA的表达,而卵巢良性肿瘤及卵巢瘤样病变组织中则无HLA-GmRNA的表达。结论卵巢恶性肿瘤组织中HLA-G mRNA及HLA-G蛋白的表达异常,提示在卵巢恶性肿瘤的发生发展中免疫抑制起到重要作用。
Objective To investigate the expression of immunosuppressive human leukocyte antigen G (HLA-G) in ovarian cancer and its clinical significance. Methods Immunohistochemical SP method was used to detect the expression of HLA-G protein in 45 cases of primary ovarian cancer and 36 cases of ovarian benign tumor by using anti-HLA-G monoclonal antibody 4H84. The expression of HLA-G mR-NA in fresh and malignant ovarian tissues was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR). Results ① Immunohistochemistry showed that the expression of HLA-G in 30 cases of ovarian malignant tumors was positive (66.67%). The expression of HLA-G in ovarian cancer was significantly higher than that in benign tissues (P <0.01) The positive rate of HLA-G between the cystadenocarcinoma, mucinous cystadenocarcinoma and endometrioid carcinoma was significantly different (P <0.05). The positive rate of HLA-G was related to age, clinical stage, pathological grade, lymph node The results of RT-PCR showed that HLA-G mRNA was expressed in ovarian cancer tissues, while the expression of HLA-G mRNA was not found in benign ovarian tumors and ovarian tumor-like tissues. Conclusion The abnormal expression of HLA-G mRNA and HLA-G protein in ovarian cancer tissues suggests that immunosuppression plays an important role in the development of ovarian cancer.