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茜素红 S 在0.01M 氢氧化钾介质中,于-0.67V 处有一个导数波.加入钙离子后,在-0.77V出现一个尖锐的新的导数波.该波的峰高与钙离子浓度有良好的线性关系.在最佳条件下,钙的最低检测限为4×10~(-8)M,浓度在7×10~(-8)～7×10~(-6)M 内与峰高成正比.本文讨论了此极谱波的机理,确定了钙-茜素红 S(ARS)配合物的组成为1∶2.本法不需任何前处理,可直接应用于血清中微量钙的测定.
Alizarin red S has a derivative wave at -0.67 V in 0.01 M potassium hydroxide medium, and a sharp new derivative wave appears at -0.77 V after addition of calcium ion.The peak height of the wave is related to the concentration of calcium ion Had a good linear relationship.The detection limit of calcium was 4 × 10 ~ (-8) M and the concentration of calcium was in the range of 7 × 10 ~ (-8) ~ 7 × 10 ~ (-6) M under the optimal conditions Which is proportional to the peak height.This paper discussed the mechanism of this polarographic wave and determined the composition of calcium-alizarin red S (ARS) complex to be 1: 2.This method does not need any pretreatment and can be directly applied to trace Determination of calcium.