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目的:探讨囊泡相关膜蛋白8(VAMP8)在结肠癌组织中的表达及其临床意义。方法:收集42例结肠癌患者癌组织及癌旁正常组织标本,用免疫组化法检测VAMP8蛋白的阳性表达率,分析其表达与结肠癌临床病理特征之间的关系;并选择其中12对标本,用q RT-PCR与Western blot法检测VAMP8 m RNA与蛋白的表达量。结果:q RT-PCR及Western blot检测显示,9对标本的结肠癌组织VAMP8 m RNA与蛋白表达量明显高于其对应的癌旁组织(均P<0.05);免疫组化结果显示,VAMP8在结肠癌组织中的阳性率明显高于癌旁组织(73.8%vs.42.9%;χ2=8.28,P<0.01),且VAMP8的表达与肿瘤的分化程度、Dukes分期及淋巴结转移情况有关(均P<0.05)。结论:VAMP8在结肠癌组织中表达上调,提示VAMP8可能通过调节自噬/溶酶体途径促进结肠癌的恶性进展与转移。
Objective: To investigate the expression of vesicular associated membrane protein 8 (VAMP8) in colon cancer and its clinical significance. Methods: 42 cases of colorectal cancer tissues and adjacent normal tissue specimens were collected. The positive expression rate of VAMP8 protein was detected by immunohistochemistry. The relationship between the expression of VEGFAMP protein and the clinicopathological features of colon cancer was analyzed. Twelve pairs of specimens The expression of VAMP8 mRNA and protein was detected by q RT-PCR and Western blot. Results: q-RT-PCR and Western blot showed that the expression of VAMP8 mRNA and protein in colon cancer tissues was significantly higher than that in the corresponding paracancerous tissues (all P <0.05). The results of immunohistochemistry showed that VAMP8 The positive rate of VEGF in colon cancer tissues was significantly higher than that in paracancer tissues (73.8% vs.42.9%; χ2 = 8.28, P <0.01), and the expression of VAMP8 was correlated with tumor differentiation, Dukes stage and lymph node metastasis <0.05). Conclusion: VAMP8 is up-regulated in colon cancer tissues, suggesting that VAMP8 may promote the malignant progression and metastasis of colon cancer by regulating the autophagy / lysosomal pathway.