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用血球凝集技术能查出食品和培养基中蜡样芽胞杆菌的肠毒素。用蜡样芽胞杆菌肠毒素皮下注射家兔所得的抗血清致敏红血球后,与蜡样芽胞杆菌的肠毒秦及培养物滤液进行凝集,其凝集价很高,纯毒素(1.5毫克/毫升)为1:262,000,培养物滤液为1:32,768。在同样条件下,用炭疽杆菌、巨大芽胞杆菌、马铃薯芽胞杆菌、枯草杆菌、蜡样芽胞杆菌变种蕈状芽胞杆菌、B.thuringiensis var. gallerae、痢疾志贺氏菌、大肠埃希氏菌、韦氏梭菌和霍乱弧菌等培养物滤液以及葡萄球菌A型和B型肠毒素作对照试验,其凝集价都很低(1:0～1:16)。将蜡样芽胞杆菌接种
The hemagglutination technique can be used to detect the enterotoxin of Bacillus cereus in food and culture medium. After the erythrocytes were sensitized with the antiserum obtained from subcutaneously injected rabbits with Bacillus cereus enterotoxin, enterotoxigenin and culture filtrates of Bacillus cereus were agglutinated and their agglutination price was high. Pure toxin (1.5 mg / ml) 1: 262,000, culture filtrate 1: 32,768. Bacillus anthracis, Bacillus megaterium, Bacillus sphaericus, Bacillus subtilis, Bacillus cereus, Bacillus thuringiensis, B. thuringiensis var. Gallerae, Shigella dysenteriae, Escherichia coli, Clostridium perfringens and Vibrio cholerae culture filtrate and Staphylococcal type A and B enterotoxins for the control test, the agglutination price is very low (1: 0 ~ 1:16). Bacillus cereus inoculation