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随机将１７４只大鼠分为Ⅰ、Ⅱ、Ⅲ组，每组５８只动物。Ⅰ组：用等渗盐水灌胃；Ⅱ组：用毒蕈匀浆液灌胃；Ⅲ组：毒蕈匀浆液灌胃同时腹腔注射山莨菪碱（６５４－２）注射液，观察肾脏生化和形态学改变。Ⅱ组灌胃后６ｈ肾皮质自由基有关指标出现明显变化，肾近曲和远曲小管出现超微结构病变，２４ｈ后出现光镜下病变，尿钠、钾排泄增加，尿渗透压降低。Ⅲ组灌胃后１２ｈ肾皮质匀浆自由基有关指标出现异常变化，肾近曲和远曲小管出现超微结构病变，晚于Ⅱ组出现；４８ｈ后出现光镜下病变，尿钠、尿钾排泄增加，尿渗透压降低。说明６５个２对毒蕈所致大鼠急性肾损伤有一定的保护作用。
A total of 174 rats were randomly divided into groups I, II and III, with 58 animals in each group. Group I: intragastrically treated with isotonic saline; group II: intragastric administration of poisonous mushroom homogenate; Group III: poisonous muscular homogenate was intraperitoneally injected with anisodamine (654-2) intraperitoneally to observe renal biochemistry and morphology change. The indexes of renal cortical free radicals changed significantly at 6h after intragastric administration in group Ⅱ. Ultrastructural lesions of renal proximal and distal convoluted tubules were observed. Lesions were observed under light microscope 24 hours later. Excretion of urine sodium, potassium and urine osmolality decreased. In group Ⅲ, there was an abnormal change of free radical in renal cortex homogenate 12h after intragastric administration, ultrastructural lesions appeared in proximal and distal convoluted tubules of rats, which were later than those in group Ⅱ. After 48h, lesions under light microscope, urine sodium, urine potassium Excretion increased, urine osmolality decreased. Explain that 65 2 have a certain protective effect on the acute kidney injury induced by toadstool in rats.