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目的:建立高效液相色谱法测定注射用头孢美唑钠的含量。方法:采用Diamonsil-C18色谱柱(4.6mm×250mm,5μm),以0.08mol·L-1醋酸铵溶液∶甲醇(80∶20)(混合后,冰醋酸调pH至6.0)为流动相,流速为1.0mL·min-1,检测波长254nm,柱温30℃;以外标法进行含量计算。结果:头孢美唑钠在0.0105~0.8400mg·mL-1浓度范围内,峰面积与浓度呈良好的线性关系,相关系数为0.9998,方法平均回收率为99.89%,中间精密度RSD为1.41%(n=6)。结论:本法简便、专属、灵敏快速,可用于注射用头孢美唑钠含量的测定。
Objective: To establish a high performance liquid chromatography for the determination of cefmetazole sodium for injection. Methods: Diamonsil-C18 column (4.6mm × 250mm, 5μm) was used as the mobile phase with 0.08mol·L-1 ammonium acetate solution: methanol (80:20) 1.0mL · min-1, detection wavelength 254nm, column temperature 30 ℃; content by external standard method. Results: Cefmetazole sodium concentration ranged from 0.0105 to 0.8400 mg · mL-1. The correlation coefficient was 0.9998. The average recovery was 99.89%, and the intermediate precision was 1.41% (RSD) n = 6). Conclusion: This method is simple, specific, sensitive and rapid and can be used for the determination of cefmetazole sodium for injection.