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本文仅就IL-2的产生能力和敏感性的测定方法介绍如下:一、人的IL-2的制备可用扁桃体或外周血淋巴细胞.常用Ficoll比重离心法,从几名正常人外周血中分得单个核细胞,再用尼龙绵吸附法分得非粘附性细胞,在含有1%FCS,5×10~(-5)M的2ME,10mM的Hepes,0.2%PHA的RPM1640培养液中培养24-48小时,采集上清液即为IL-2的粗制品.如在培养液中加入淋巴母细胞传代株作为同种异型抗原刺激,或加入5-10μg/ml的PMA(乙酸肉豆寇佛波醇)、5×10~(-6)M消炎痛(indo methacin)都可增加IL-2的产生能力.最
This article only on the determination of IL-2 production capacity and sensitivity as follows: First, the preparation of human IL-2 available tonsils or peripheral blood lymphocytes. Ficoll gravity centrifugation commonly used method, from several normal peripheral blood The mononuclear cells were obtained and then non-adherent cells were separated by nylon adsorption and cultured in RPM1640 medium containing 1% FCS, 5 × 10 -5 M in 2ME, 10 mM Hepes, 0.2% PHA 24-48 hours, the supernatant was collected crude IL-2 products, such as adding lymphoblastoid cell line in the culture medium as an alloantigen-stimulated or added 5-10μg / ml of PMA Alcohol), 5 × 10 ~ (-6) M indo methacin can increase the production of IL-2.