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目的建立诊断肝豆状核变性患者的生物样品中微量铜的石墨炉原子吸收检测方法。方法尿液和血清样品经1%HNO3稀释后,采用石墨炉原子吸收光谱标准曲线法直接测定。结果线性范围0～200μg/L,相关系数0.9997,方法检出限为1.4μg/L,回收率96.7%～99.2%,相对标准偏差1.0%～1.7%,经冻干人尿标样(GBW 09102)对照测定,其结果在允许范围内,相对误差为-1.2%。结论采用GFAAS法测定生物样品中铜,方法可靠、准确、灵敏,能适用于肝豆状核变性诊断中生物样品微量铜检测的要求。
Objective To establish a method for the determination of trace copper in biological samples of patients with hepatolenticular degeneration by graphite furnace atomic absorption spectrometry. Methods Urine and serum samples were diluted with 1% HNO3 and determined directly by graphite furnace atomic absorption spectrometry (CLIA) standard curve method. Results The linear range was from 0 to 200 μg / L with a correlation coefficient of 0.9997. The detection limit was 1.4 μg / L, the recovery was 96.7% -99.2% and the relative standard deviation was 1.0% -1.7%. The lyophilized human urine sample (GBW 09102 ) Control test, the result is within the allowable range, the relative error is -1.2%. Conclusion The determination of copper in biological samples by GFAAS method is reliable, accurate and sensitive and can be applied to the detection of trace copper in biological samples during the diagnosis of Wilson’s disease.