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采用荧光探针结合计算机图像处理技术测定幼年、成年、老年ＳＨＲ心肌单细胞内游离Ｃａ~（２＋）浓度，采用高压液相色谱法测定幼年、成年、老年ＳＨＫ心肌去甲肾上腺素（ＮＥ）含量。结果显示随着年龄增加，ＳＨＲ心肌细胞内游离Ｃａ~（２＋）增加，而心肌组织ＮＥ含量下降。与老年ＷＫＹ大鼠比较，老年ＳＨＲ心肌细胞内Ｃａ（２＋）含量较高（Ｐ＜０．０１），而其心肌组织ＮＥ含量却较少（Ｐ＜０．０５）。本研究提示心肌组织ＮＥ与ＳＨＲ的血压升高及心肌细胞内游离Ｃａ~（２＋）增多无直接关系。＊Ｐ＜０．０１，ａ．ｖｓ．ＳＨＲ２ｍ，ｂ．ｖｓＳＨＲ６ｍ，ｃ．ｖｓ．ＳＨＲ１２ｍ３不同年龄阶段ＳＨＲ心肌组织ＮＥ含量ＳＨＲ的年龄越大，其心肌组织ＮＥ的含量越少，各年龄组之间均有显著差异（Ｐ＜０．０１）。与老年ＷＫＹ大鼠比较．老年ＳＨＲ是心肌组织ＮＥ含量明显降低（Ｐ＜０．０５）（Ｆｉｇ３）。Ｆｉｇ３ＮＥｃｏｎｔｅｎｔｓｉｎｃａｒｄｉａｃｔｉｓｓｕｅｓＩｎＳＨＲｓｏｆｖａｒｉｏｕｓａｇｅｓａｎｄｅｌｄｅｒＷＫＹｒａｔｓ：Ｐ＜０．０５．＊：Ｐ＜０．０１，ａ．ｖｓ．ＳＨＲ２ｍ，ｂ．ｖｓＳＨＲ６ｍ．ｃ．ｖｓＳＨＲ１２ｍ．ＤＩＳＣＵＳＳＩＯＮＦｕｒａ－２能选择性地
Fluorescent probes combined with computer image processing were used to measure intracellular free Ca 2+ concentration in isolated SHR myocardium of young, adult and old people. The content of norepinephrine (NE) in young, adult and aged SHK myocardium was measured by high pressure liquid chromatography . The results showed that as age increased, intracellular free Ca2 + increased in myocardial cells of SHR, whereas the content of NE in myocardium decreased. Compared with aged WKY rats, the contents of intracellular calcium (Ca 2+) in elderly SHR were higher (P <0.01), while the content of NE in myocardium was lower (P <0.05). This study suggests that there is no direct relationship between myocardial NE and SHR blood pressure and intracellular free Ca 2+. * P <0.01, a. vs. SHR2m, b. vsSHR6m, c. vs. SHR12m3 SHR myocardial tissue in different age groups SHR the greater the age, the less the content of myocardial NE, significant differences between the various age groups (P <0.01). Compared with aged WKY rats. The content of NE in the aged SHR was significantly decreased (P <0.05) (Fig3). Fig3NEcontentsincardiactissuesInSHRsofvariousagesandelderWKYrats: P <0.05. *: P <0.01, a. vs. SHR2m, b. vsSHR6m. c. vsSHR12m. DISCUSSIONFura-2 can optionally