河南省甘薯茎腐病的分离与鉴定

• 作 者: 秦素研 黄立飞 葛昌斌 刘志坚 张勇跃 李建国 房伯平
• 机 构: 漯河市农业科学院,广东省农业科学院作物研究所,
• 出 处: 作物杂志
• 发表日期: 2013年06期
• 关键词: 甘薯 柯赫氏法则 茎腐病 甘薯病害 菊欧文氏菌 分子鉴定 Erwinia 病原菌 水浸状 河南省襄城县

2012年在河南省襄城县甘薯产区发现了一种引起甘薯快速死苗的甘薯病害,主要症状表现为叶片变黄,茎基部呈黑色水浸状腐烂,并逐渐沿茎枝向顶端腐烂,后整株倒伏、死亡。从病地采集受害甘薯茎蔓后分离纯化获得了病原菌,经柯赫氏法则验证及16S rDNA序列分析,鉴定其为菊欧文氏菌(Erwinia chrysanthemi)甘薯茎腐病。这是河南省首次发现该病害。

In 2012, sweet potato disease was found in the sweet potato producing area of ​​Xiangcheng County, Henan Province. The main symptom of the disease was the yellowing of the leaves, the rotting of the base of the stems in black and gradually decaying along the branches of the stems, After the whole lodging, death. Pathogenic bacteria were isolated and purified from the diseased land of sweet and sour stems and vines, and identified as Erwinia chrysanthemi sweet potato stalk rot by Koch’s law and 16S rDNA sequence analysis. This is the first time that the disease was found in Henan Province.