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为评价转鼠酪氨酸羟化酶（TH）基因的成肌细胞脑内移植对偏侧帕金森病（PD）大鼠模型纹状体区多巴胺及代谢产物含量的影响，应用6－羟多巴胺损毁SD大鼠单侧黑质制备偏侧帕金森病臣模型。模型稳定必个月后，移植转TH基因的成肌细胞（n＝24）或未转基国的成肌细胞（n＝10）于偏侧PD鼠损毁侧纹状体。移植治疗后6个月，用高效液相色谱电化学法（HPLC－ECD）检测偏侧PD鼠模型纹状体区多巴胺、3，4－二羟苯乙酸（DOPAC）以及高香草酸（HVA）含量。结果转TH基因成肌细胞植入组PD鼠纹状体区多巴胺及代谢产物含量明显增高（P＜0．01），其中多巴胺含量从治疗前平均39．20Pg／mg提高至治疗后的985．71Pg／mg，相当于正常侧纹状体的49．99％．对照组植入未转基因的成肌细胞后纹状体多巴胺及代谢产物含量无明显变化（P＞0．05）。可见转鼠TH基因的成肌细胞脑内移植能够部分改善偏侧PD鼠模型纹状体区多巴胺的缺乏，成肌细胞是PD基因治疗的合适靶细胞之一。
To evaluate the effect of transplanted myoblasts transfected with tyrosine hydroxylase (TH) gene on dopamine and metabolites in striatum of rats with hemiparkinsonism (PD), we used 6-hydroxydopamine Unilateral unilateral substantia nigra of SD rats was damaged to prepare a model of Parkinson ’s disease. After one month of model stabilization, myoblasts transfected with TH gene (n = 24) or non-basal myoblasts (n = 10) impaired lateral striatum in the basilar PD rats. Six months after transplantation, dopamine, DOPAC and HVA were detected by HPLC-ECD in the striatum of PD rats. content. Results The contents of dopamine and metabolites in the striatum of PD rats were significantly increased (P <0.01), and the content of dopamine increased from 39.20 Pg / mg before treatment to 985 after treatment. 71 Pg / mg, equivalent to 49.99% of the normal lateral striatum. There was no significant change in the contents of dopamine and metabolites in the control group after implantation of non-transgenic myoblasts (P> 0.05). Transplantation of myoblasts transplanted with TH gene into the rat brain can partially alleviate the lack of dopamine in the striatum in the PD model. Myoblasts are one of the suitable target cells for PD gene therapy.