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建立了超高效液相色谱串联三重四极杆质谱(UHPLC-MS/MS)测定大鼠血浆中的芍药苷含量的分析方法,并用于芍药苷药动学研究。采用UHPLC-MS/MS电喷雾电离源(ESI),负离子模式,多反应监测(MRM)法定量分析芍药苷,离子对为m/z 479.1→m/z 121.0和m/z 479.1→m/z 327。计算芍药苷血浆质量浓度,采用PK Solutions 2.0软件计算药动学参数。大鼠血浆中芍药苷在0.01~2μg/mL范围内线性良好,定量限为0.01μg/mL。日内和日间RSD均<11%,准确度均在±15%之间。低、中、高3个质量浓度下绝对回收率均>70%。大鼠腹腔注射20 mg/kg芍药苷后的主要药动学参数c_(max)为(14.28±1.76)μg/mL,T_(1/2)E_(phase)为(176.11±23.47)min,AUC_(0-t)为(1193.30±213.70)ng·min/mL。
A method for the determination of paeoniflorin in rat plasma by ultra performance liquid chromatography tandem triple-quadrupole mass spectrometry (UHPLC-MS / MS) has been developed and applied to the pharmacokinetics of paeoniflorin. Paeoniflorin was quantitatively analyzed by UHPLC-MS / MS electrospray ionization source (ESI), negative ion mode and multiple reaction monitoring (MRM). The ion pair was m / z 479.1 → m / z 121.0 and m / z 479.1 → m / 327. Paeoniflorin plasma concentration was calculated and pharmacokinetic parameters were calculated using PK Solutions 2.0 software. Paeoniflorin in rat plasma had a good linearity in the range of 0.01-2 μg / mL with a limit of quantitation of 0.01 μg / mL. The RSDs were both <11% for day and day, with an accuracy of ± 15%. The average recoveries of three kinds of low, medium and high concentration were> 70%. The main pharmacokinetic parameters of rat after intraperitoneal injection of 20 mg / kg paeoniflorin were (14.28 ± 1.76) μg / mL and T_ (1/2) E phase (176.11 ± 23.47) min and AUC_ (0-t) was (1193.30 ± 213.70) ng · min / mL.