外周血单核细胞中丙型肝炎病毒RNA正负链检测的临床意义

• 作 者: 吴海滨 李智伟 李颖
• 机 构: 中国医科大学附属第二医院传染科
• 出 处: 世界华人消化杂志
• 发表日期: 1999年03期
• 关键词: 外周血单核细胞 丙型肝炎病毒 负链 检测

目的通过对外周血单核细胞（ＰＢＭＣ）中ＨＣＶＲＮＡ正负链的检测，来探讨其与丙型肝炎慢性化及干扰素治疗的关系．方法慢性丙型肝炎患者４０例，其中干扰素治疗者１０例，分离血清及ＰＢＭＣ．异硫氰酸胍－酚－氯仿抽提法提取ＨＣＶＲＮＡ，应用逆转录－巢式ＰＣＲ技术检测ＨＣＶＲＮＡ正负链．结果血清正链ＨＣＶＲＮＡ阳性率为６７５％，但负链均为阴性，ＰＢＭＣ中正链ＨＣＶＲＮＡ阳性率为５７５％，负链的阳性率为３５０％．其中３例患者血清中正链ＨＣＶＲＮＡ为阴性，而ＰＢＭＣ中为阳性．１０例干扰素治疗者在治疗结束时血清正链ＨＣＶＲＮＡ６０％转阴，ＰＢＭＣ中负链ＨＣＶＲＮＡ８０％转阴，而正链仅３７５％转阴．结论ＨＣＶ能在ＰＢＭＣ中存在和复制，这可能是导致丙型肝炎易发生慢性化的原因之一．ＰＢＭＣ中ＨＣＶＲＮＡ正负链的检测对于临床判断干扰素的疗效及预后有重要意义

OBJECTIVE: To detect the relationship between positive and negative HCVRNA in peripheral blood mononuclear cells (PBMCs) by chronic HCV infection and interferon therapy. Methods 40 cases of chronic hepatitis C patients, including 10 cases of interferon treatment, serum and PBMC were separated. HCV RNA was extracted by guanidinium isothiocyanate - phenol - chloroform extraction, and positive and negative HCV RNA were detected by reverse transcription - nested PCR. Results The positive rate of positive HCVRNA in serum was 675%, but the negative ones were negative. The positive rate of positive HCV RNA in PBMC was 575% and negative rate was 350%. Serum positive strand HCVRNA was negative in 3 of the patients and positive in PBMC. 10 cases of interferon treatment at the end of the serum positive strand HCV RNA 60% negative, PBMC negative strand HCV RNA 80% negative, while the positive chain only 37  5% negative. Conclusion HCV can exist and replicate in PBMC, which may be one of the reasons leading to the chronicity of hepatitis C. Detection of PBMC HCVRNA positive and negative chain for the clinical judgment of interferon efficacy and prognosis of great significance