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造血干细胞因子（ＳＣＦ）是一种多功能造血生长因子，与其它造血生长因子协同作用，刺激不同分化阶段的造血细胞增殖和分化。本文采用ＲＴ－ＰＣＲ技术从新生ＢＡＬＢ／Ｃ小鼠胸腺组织克隆了ＳＣＦ膜外功能区ｃＤＮＡ，进而在大肠杆菌表达出具有生物学活性的重组蛋白。
Hematopoietic stem cell factor (SCF) is a multifunctional hematopoietic growth factor that synergizes with other hematopoietic growth factors to stimulate hematopoietic cell proliferation and differentiation at different stages of differentiation. In this paper, cDNA of SCF extracellular domain was cloned from neonatal BALB / C mouse thymus tissue by RT-PCR, and the recombinant protein with biological activity was expressed in E. coli.