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目的 评价大黄素 (3 甲 1,6 ,8 三羟蒽醌 ) ,一种植物雌激素对去卵巢大鼠和经 βAP1 4 0 处理的培养的神经元的作用。 方法 应用动物行为学和原代皮层神经元培养方法对大黄素进行评价。通过Morris水迷宫评价去卵巢造成的大鼠智力行为学变化和饲喂大黄素 70d大鼠的行为学变化。培养的神经元分别用 β淀粉样蛋白1 4 0 ,β淀粉样蛋白1 4 0 + 17 β 雌二醇 ,和 β淀粉样蛋白1 4 0 +大黄素处理。用流式细胞仪检测皮层神经元和培养细胞中的凋亡细胞。 结果 去卵巢大鼠的时间逃避潜伏期及距离逃避潜伏期均长于对照大鼠和经大黄素处理大鼠。去卵巢大鼠神经元和经βAP1 4 0 处理的培养神经元的凋亡百分比及荧光强度均高于饲喂大黄素动物神经元及经大黄素处理培养神经元。结论 本研究支持这样的工作假说 :大黄素能保护去卵巢大鼠及培养的神经元抗 β淀粉样蛋白1 4 0 的毒性作用
Objectives To evaluate the effects of emodin (3 alpha 1,6,8 trisoxine), a phytoestrogen, on ovariectomized rats and cultured neurons treated with [beta]AP1 4 0 . Methods Emodin was evaluated by animal behavior and primary cortical neuron culture methods. Evaluation of ovariectomized mental behavior in rats and behavioral changes in emodin 70 d rats were evaluated by Morris water maze. The cultured neurons were treated with β amyloid 140, β amyloid 14 0 + 17 β estradiol, and β amyloid 1 4 0 + emodin. Flow cytometry was used to detect apoptotic cells in cortical neurons and cultured cells. Results The time of escape avoidance and the distance to escape were longer in the ovariectomized rats than in the control rats and emodin treated rats. The apoptosis percentage and fluorescence intensity of neurons in ovariectomized rats and those treated with βAP140 were higher than those of neurons fed emodin and neurons treated with emodin. Conclusion This study supports the hypothesis that emodin protects ovariectomized rat and cultured neurons against the toxic effects of β-amyloid protein 140