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目的：建立时间分辨荧光核酸杂交分析体系并应用于临床乙型肝炎病毒（ＨＢＶ）ＤＮＡ检测。方法：采用本室自制螯合剂异硫氰酸苯基－ＥＤＴＡ制备铕离子标记的链霉亲和素（Ｅｕ３＋－ＳＡ），抽提患者血清中的ＤＮＡ，包被于聚苯乙烯微滴板中，与生物素化ＨＢＶＤＮＡ杂交后，以Ｅｕ３＋－ＳＡ为检测物，应用时间分辨荧光技术检测血清样本中的ＨＢＶＤＮＡ。结果：本实验共检查了４３例临床血清样本，在２９例ＨＢｅＡｇ阳性病例中共检出２５例ＨＢＶＤＮＡ阳性，阳性率为８６．２１％；在１４例ＨＢｅＡｂ阳性病例中检出２例ＨＢＶＤＮＡ阳性，阳性率为１４．２９％。结论：本方法快速、简便、易自动化处理，是一项值得推广的非放射性分析检测技术。
Objective: To establish a time-resolved fluorescent nucleic acid hybridization analysis system and apply it to the detection of clinical hepatitis B virus (HBV) DNA. Methods: Europium ion-labeled streptavidin (Eu3 + -SA) was prepared by isothiocyanate phenyl-EDTA. The DNA in patient’s serum was extracted and coated in polystyrene microtitre plate , After hybridization with biotinylated HBVDNA, Eu3 + -SA as the detection material, the application of time-resolved fluorescence detection of serum samples HBVDNA. Results: A total of 43 clinical serum samples were examined in this study. Totally 25 HBVDNA positive cases were detected in 29 HBeAg positive cases, the positive rate was 86.21%. In 14 HBeAb positive cases, 2 HBVDNA positive and positive The rate was 14.29%. Conclusion: The method is rapid, simple and easy to automate. It is a non-radioactive analysis and detection technique worthy of promotion.