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目的:探讨雌激素保护脑卒中后束缚应激(PSR)大鼠作用与降钙素基因相关肽(CGRP)的相关性。方法:雌性SD大鼠50只,经Open-Field实验评分后随机分成对照组、脑卒中组、束缚组、PSR组及雌激素治疗组,并建立相应模型。观察各组大鼠不同阶段行为学改变,并运用特异性放射免疫分析方法测定各组大鼠外周血、脊髓及前额叶的CGRP样免疫活性(CGRP-LI)物质,观察各组间CGRP-LI的改变。结果:与对照组比较,PSR大鼠Open-Field实验中水平和垂直得分明显减少,外周及中枢CGRP-LI含量明显降低;与PSR组比较,雌激素治疗组大鼠Open-Field实验中得分增加,外周血、脊髓及前额叶CGRP-LI含量较PSR组升高。结论:CGRP水平的上调可能是雌激素对PSR大鼠的保护作用之一。
Objective: To investigate the correlation between estrogen and cGRP in post-stroke restraint stress (PSR) rats. Methods: Fifty female SD rats were randomly divided into control group, stroke group, restraint group, PSR group and estrogen treatment group according to Open-Field test. The behavioral changes at different stages of the rats in each group were observed. CGRP-LI substances in the peripheral blood, spinal cord and prefrontal cortex were detected by radioimmunoassay. CGRP-LI Change. Results: Compared with the control group, the open-field test of PSR rats significantly reduced the horizontal and vertical scores and the content of CGRP-LI in peripheral and central cortex decreased significantly. Compared with the PSR group, the score of open-field test in estrogen-treated rats increased , And the levels of CGRP-LI in peripheral blood, spinal cord and prefrontal lobe were higher than those in PSR group. Conclusion: The upregulation of CGRP may be one of the protective effects of estrogen on PSR rats.