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目的 观察端粒酶基因在乳腺癌前病变 乳腺导管非典型增生及乳腺癌中的表达 ,探讨其与恶性转化的关系。方法 应用原位杂交方法评价端粒酶基因hTR和hTRT在 5 0例乳腺增生组织 (其中乳腺单纯性增生 6例 ,轻度非典型增生 9例 ,中度非典型增生 12例 ,重度非典型增生 2 3例 )及 2 6例乳腺癌中的表达。结果 在乳腺导管单纯性增生组织中端粒酶基因 (hTR、hTRTmRNA)呈弱表达 (16 6 % )或表达阴性。在乳腺导管轻、中度非典型增生组织中端粒酶基因 (hTR、hTRTmRNA)呈弱表达 (2 2 2 % ,11 1% ;33 3% ,2 5 0 % ) ,在乳腺导管重度非典型增生组织中端粒酶基因 (hTR、hTRTmRNA)表达增强 (6 0 9% ,5 2 1% ) ,在乳腺癌组织中端粒酶基因(hTR、hTRTmRNA)呈较强阳性表达 (88 5 % ,80 8% )。乳腺导管重度非典型增生组织中的端粒酶基因表达与在轻中度非典型增生、乳腺浸润性导管癌组织中的表达比较差异显著 (P <0 0 5 ) ,但与导管内癌表达无显著差异(P >0 0 5 )。结论 端粒酶基因hTR、hTRT的表达与乳腺导管非典型增生细胞的恶性转化有关 ,端粒酶的重新激活可能在乳腺癌的组织发生中起作用
Objective To observe the expression of telomerase gene in mammary duct atypical hyperplasia and breast cancer in the precancerous breast lesions, and to investigate its relationship with malignant transformation. Methods In situ hybridization was used to evaluate the hTR and hTRT genes in 50 cases of breast hyperplasia (6 cases of simple breast hyperplasia, 9 cases of mild atypical hyperplasia, 12 cases of moderate dysplasia, severe dysplasia 23 cases) and 26 cases of breast cancer. Results The telomerase gene (hTR, hTRT mRNA) was weakly expressed (16 6%) or negative in the ductal hyperplasia. Telomerase gene (hTR, hTRT mRNA) was weakly expressed in light and moderate atypical hyperplasia of mammary ducts (22%, 11%; 33%, 25%), and atypical ductal atypicality was atypical. The expression of telomerase gene (hTR, hTRT mRNA) was increased in hyperplasia tissues (60%, 52%), and the telomerase gene (hTR, hTRT mRNA) was strongly positive (88.5 %) in breast cancer tissues. 80 8%). The expression of telomerase gene in severe atypical hyperplasia of breast duct was significantly different from that in mild to moderate atypical hyperplasia and breast invasive ductal carcinoma (P < 0.05), but it was not associated with intraductal carcinoma. Significant difference (P > 0 0 5). Conclusion The expression of telomerase gene hTR and hTRT is related to the malignant transformation of atypical hyperplasia of breast duct. The reactivation of telomerase may play a role in the histogenesis of breast cancer.