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目的通过观察中药肝脾调补方对日本血吸虫病肝损伤模型小鼠肝功能指标及有关细胞因子表达水平的影响,研究其对小鼠日本血吸虫病肝损伤的保护作用及其机制。方法 72只昆明小鼠随机分为A正常组、B模型组、C肝脾调补方组,用日本血吸虫尾蚴皮肤敷贴法感染小鼠,构建日本血吸虫病肝损伤模型,于8周、12周分别随机抽取10只小鼠,自小鼠眼眶静脉丛取血和刨杀留取肝脏标本,观察其肝脏组织形态,ELISA检测ALT、AST活性,采用免疫组织化学法检测TNF-α和TGF-β1的表达变化。结果感染8周和12周,B组的ALT和AST水平与同期A组比较差异具有统计学意义(P﹤0.05),C组的ALT和AST水平较B组显著降低(P﹤0.05);B组在感染8周时,可见大量急性虫卵肉芽肿病变,汇管区纤维组织增生,周围有大量炎性细胞浸润;部分肉芽中心出现坏死,形成嗜酸性脓肿,12周时,肝细胞水肿明显,肝内汇管区虫卵肉芽肿明显增多,周围可见明显的纤维增生,C组虫卵肉芽肿明显减少,肉芽肿周围和汇管区纤维程度明显减轻;B组在感染8周时TNF-α表达明显增加,12周时TGF-β1的表达明显增加,C组两种细胞因子均低于B组,差异具有统计学意义(P﹤0.05)。结论肝脾调补方对日本血吸虫病所致肝损伤有保护作用,其机制可能与抑制TNF-α和TGF-β1的表达有关。
Objective To observe the effect of Ganpi Tiaobu Recipe on liver function and related cytokine expression in mice with schistosomiasis japonica hepatic injury model and to study its protective effect on mice with schistosomiasis japonicum liver injury and its mechanism. Methods Seventy-two Kunming mice were randomly divided into A normal group, B model group and C group. The mice were infected with Schistosoma japonicum cercarial skin patch to construct a model of hepatic injury of Schistosoma japonicum at 8 weeks, 12 Totally 10 mice were randomly selected from the orbital venous plexus of the mice to take blood and sharks to take the liver specimens, observe the liver morphology, ELISA test ALT, AST activity, immunohistochemical detection of TNF-α and TGF- β1 expression changes. Results The levels of ALT and AST in group B were significantly different from those in group A (P <0.05) at 8 and 12 weeks of infection. The levels of ALT and AST in group C were significantly lower than those in group B (P <0.05); B At 8 weeks of infection, a large number of acute ova granuloma lesions were found in the group. The fibrous tissue of the portal area was hyperplastic with a large number of inflammatory cells infiltrating around. Some of the granulation centers were necrotic and eosinophilic abscess was formed. At 12 weeks, Intrahepatic portal area egg granuloma was significantly increased around the obvious fibrous proliferation, C group of egg granuloma was significantly reduced, granuloma and portal area around the fiber was significantly reduced; group B at 8 weeks of infection TNF-α expression was significantly (P <0.05). At 12 weeks, the expression of TGF-β1 was significantly increased. The two cytokines in group C were lower than those in group B, the difference was statistically significant (P <0.05). Conclusion Liver, spleen and tonifying prescription can protect liver from schistosomiasis japonica, which may be related to the inhibition of the expression of TNF-α and TGF-β1.