乙型肝炎患者HBV前C区变异及临床意义

• 作 者: 胡耀仁 唐锡尔 应豪 周元龙 翁伦华 徐陈槐
• 机 构: 宁波市传染病医院,浙江大学传染病研究所
• 出 处: 宁波医学
• 发表日期: 1999年03期
• 关键词: 乙型肝炎 HBV前C区 变异率 聚合酶链反应 重型肝炎 急性肝炎 慢性肝炎 病毒携带者 位点变异 片段长度

目的研究乙型肝炎病毒（ＨＢＶ）前Ｃ区变异的临床意义。方法应用错配ＰＣＲ限制片段长度多态性分析，检测ＨＢＶ前Ｃ区１８９６位突变。结果６２０例乙型肝炎患者ＨＢＶ前Ｃ区１８９６位变异２６４例，变异率４２．５８％。在急性肝炎、慢性轻度、慢性中度、慢性重度、重型肝炎、肝硬化中，变异率分别为１２．５％、３５．０％、５５．９４％、４５．２６％、５４．７２％、４８．７６％。年龄、性别与变异无关，病程＞５年、谷丙转氨酶（ＡＬＴ）异常、抗ＨＢｅ（＋）者变异率均明显增高。结论ＨＢＶ前Ｃ区变异与肝病严重性有一定关系。

Objective To study the clinical significance of pre-C mutation in Hepatitis B virus (HBV). Methods Mismatched PCR restriction fragment length polymorphism analysis, pre-HBV precore 1896 mutation was detected. Results 620 cases of hepatitis B patients with pre-HBV precinct 1896 mutations in 264 cases, the mutation rate of 42.58%. The rates of mutation were 12.5%, 35.0%, 55.94%, 45.26% and 54.72% in acute hepatitis, chronic mild, chronic moderate, chronic severe, severe hepatitis and cirrhosis respectively. , 48.76%. Age, gender and mutation had nothing to do, duration of disease> 5 years, alanine aminotransferase (ALT) abnormalities, anti- HBe (+) were significantly higher mutation rates. Conclusion The pre-HBV mutation has some relationship with the severity of liver disease.