论文部分内容阅读
目的 了解小儿急性白血病的免疫病理机制 ,探讨TH1 /TH2型细胞因子平衡性在发病中的作用 ,为临床免疫防治白血病提供理论依据。方法 应用双抗体夹心酶联免疫吸附法检测了 39例急性白血病患儿化疗前后血清IL 1 0和IFN γ水平 ,并进行相关性分析。结果 白血病患儿治疗前或复发时血清IL 1 0明显升高 ,IFN γ明显降低 ,治疗后病情缓解时IL 1 0水平下降而IFN γ水平升高 ,两者呈负相关关系。结论 小儿白血病的免疫病理机制与TH1 /TH2细胞功能紊乱有密切关系 ,血清IL 1 0、IFN γ水平变化可作为监测小儿白血病治疗反应的一种手段。
Objective To understand the immunopathological mechanism of pediatric acute leukemia and to explore the role of TH1 / TH2 cytokine balance in the pathogenesis and to provide a theoretical basis for clinical immunological prevention and treatment of leukemia. Methods Serum levels of IL-10 and IFN-γ in 39 children with acute leukemia before and after chemotherapy were measured by double antibody sandwich enzyme-linked immunosorbent assay and their correlations were analyzed. Results The levels of serum IL-10 in patients with leukemia before relapse or at relapse were significantly higher than those in patients with relapsed leukemia. The levels of IL-10 decreased and IFN-γ increased after treatment, and there was a negative correlation between them. Conclusion The immunopathological mechanism of childhood leukemia is closely related to the dysfunction of TH1 / TH2 cells. The changes of serum IL-10 and IFN-γ level may be used as a means to monitor the therapeutic response of childhood leukemia.