论文部分内容阅读
[目的]探讨云芝氯仿萃取物对肝癌HepG-2细胞的凋亡作用.[方法]采用MTT方法检测不同浓度云芝氯仿萃取物对肝癌HepG-2细胞生存抑制率的影响;应用HE染色方法观察不同质量浓度云芝氯仿萃取物对肝癌HepG-2细胞形态学变化的影响.[结果]MTT方法观察结果显示,云芝氯仿萃取物可抑制肝癌HepG-2细胞的增殖,随着时间的延长和药物浓度的增加,抑制作用越明显,当药物浓度为200mg/L、作用时间为48h时对HepG-2细胞的抑制率为52.3%,接近半数抑制浓度.HE染色结果显示,云芝氯仿萃取物处理的肝癌HepG-2细胞,与对照组相比细胞间距增大、细胞变小、失去原有形态、细胞核皱缩、染色变深、细胞碎裂、可见凋亡小体,并且随着时间的延长和剂量的增加,凋亡程度越严重.[结论]云芝氯仿萃取物可促进肝癌HepG2细胞的凋亡.
[Objective] To explore the apoptosis of hepatocellular carcinoma HepG-2 cells treated with chlorogenic acid chloroform extract. [Methods] MTT assay was used to determine the effect of different concentrations of chloroform extract on the survival inhibition rate of HepG-2 cells. HE staining The effects of different concentrations of chloroform-chloroform extract on the morphological changes of HepG-2 cells were observed. [Results] The results of MTT assay showed that the chloroform extract of Yunyi could inhibit the proliferation of HepG-2 cells. With the increase of time And the increase of drug concentration, the more obvious the inhibitory effect was.When the drug concentration was 200mg / L and the action time was 48h, the inhibition rate of HepG-2 cells was 52.3%, which was close to the half inhibitory concentration.The results of HE staining showed that, Compared with the control group, the HepG-2 cells treated with HepG-2 and HepG-2 cells showed smaller cell size, lost their original morphology, shrinkage of the nucleus, deeper staining, cell fragmentation, apoptotic bodies, Prolonged and dose increased, the more serious the degree of apoptosis was. [Conclusion] The chloroform extract of Yunzhi can promote the apoptosis of HepG2 cells.