论文部分内容阅读
目的:比较香加皮中4种强心苷类成分的强心作用并确定其时效和量效关系。方法:采用Langendor-ff离体心脏灌流法,待实验心脏稳定后给予受试药,记录并比较心率(HR)、左心室收缩压(LVSP)、左心室舒张末压(LVEDP)、dp/dtmax、dp/dtmin、等心功能指标。结果:香加皮中4种强心苷类成分均能提高大鼠离体心脏的心肌收缩力和左心室收缩压,随着各自剂量的逐渐增大,强心作用逐渐增强,到达某一剂量后,强心作用开始降低。与给药前相比,4种强心苷成分在给药后HR、LVSP、dp/dtmax均有显著性的改变(P<0.05)。在时效关系研究中达峰顺序依次为xysmalogenin、杠柳毒苷苷元、杠柳毒苷、强心苷G1。在量效关系研究中杠柳毒苷的最大作用浓度为0.15mg/只,而杠柳毒苷苷元的最大作用浓度为0.30mg/只。结论:4种强心苷类成分都能增强大鼠离体心脏功能,均兼备正性肌力作用和负性频率作用。
Objective: To compare the cardiac effects of four cardiac glycosides in Xiangkapi and to determine the relationship between the effects of aging and dose-response. Methods: Langendor-ff was used in this study. After the heart was stabilized, the test drug was given and the heart rate (HR), left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP), dp / dtmax , Dp / dtmin, and other cardiac function indicators. Results: Four kinds of cardiac glycosides in Xiangkapi can improve myocardial contractility and left ventricular systolic pressure in isolated rat hearts. With the gradual increase of their respective doses, their cardiac function gradually increased. After reaching a certain dose After cardiac function began to decrease. Compared with before administration, the four kinds of cardiac glycosides showed significant changes in HR, LVSP and dp / dtmax after administration (P <0.05). In the study of the relationship between the aging peak order followed by xysmalogenin, peridin toxin, perlithin, cardiac glycosides G1. In the dose-response relationship study, the maximum acting concentration of perlithin was 0.15 mg / tablet, and the maximum acting concentration of peridin toxin was 0.30 mg / tablet. CONCLUSION: All the four kinds of cardiac glycosides can enhance cardiac function in isolated rats, both of them have positive inotropic and negative frequency effects.