茶树优质资源遗传稳定性的RAPD分析

• 作 者: 陈亮 虞富莲 杨亚军 陈大明 徐昌杰 高其康
• 机 构: 中国农业科学院茶叶研究所,浙江大学园艺系
• 出 处: 茶叶科学
• 发表日期: 1999年01期
• 关键词: 茶树品种 RAPD分析 遗传稳定性 扦插繁殖 扩增产物 举岩茶 随机引物 随机扩增多态性 群体品种 遗传多样性

应用随机扩增多态性ＤＮＡ（ＲＡＰＤ）技术，对汝城早芽、蓝标１号、云桂大叶、日铸茶、举岩茶等５份茶树优质资源及其扦插繁殖后代的遗传稳定性进行了分析。结果表明，基因组ＤＮＡ的平均得率为７７２μｇ／ｇ鲜重，Ａ２６０／Ａ２８０在１６９～１８３之间，２０个随机引物在５份资源中共扩增出４１２个位点，平均每个引物２０６个，每份资源８２４个位点，扩增产物片段大小约在０４～２５ｋｂ之间，多态性程度达８４９％。２０个随机引物在５份优质资源及其扦插繁殖后代的ＲＡＰＤ扩增结果显示，无论是谱带的类型还是谱带的强弱，资源内是一致的，从而在ＤＮＡ分子水平上证明了扦插繁殖的遗传稳定性。

Random amplified polymorphic DNA (RAPD) technique was used to analyze the genetic stability of five high quality tea resources and the progenies of cutting propagation of Rucheng early bud, blue mark No.1, Yungui big leaf, Rugao tea, Sexuality was analyzed. The results showed that the average yield of genomic DNA was 772 μg / g fresh weight, A260 / A280 was between 169 and 183, and that of 20 random primers amplified 412 loci in 5 accessions, with an average of 20  6 primers, 82  4 loci per copy. The size of amplified products was about 04 ~ 2  5 kb, and the degree of polymorphism was 849%. The results of RAPD amplification of 20 random primers in 5 high-quality resources and their progeny cuttings show that both the types of bands and the strength of the bands are consistent within the resources, thus demonstrating cutting propagation at the DNA molecular level The genetic stability.