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目的研究甲醛、乙醛、丙醛、丁醛、己醛、戊醛对脱细胞-核DNA的影响。方法将甲醛、乙醛、丙醛、丁醛、己醛、戊醛用缓冲液分别配制浓度为10 mmol/L的溶液作为受试物;用羟自由基损伤的脱细胞-核DNA断片模型检测受试物与DNA交互作用的能力;用未经羟自由基处理的脱细胞-核DNA模型检测受试物导致DNA断裂的程度。两种模型均为每组6个平行板,处理时间均为60 min,用彗星实验检测DNA拖尾情况,每张板用彗星分析软件(Comet A1.0)分析50个脱细胞-核DNA,组间差异用SPSS 18.0软件进行统计学分析。结果经甲醛处理的脱细胞-核DNA断片模型无DNA片段泳出;经乙醛处理的脱细胞-核DNA断片模型中DNA片段泳出量明显低于对照组;其他醛类对脱细胞-核DNA断片模型的DNA断片泳出量与对照组比较差异无统计学意义;所有醛类均未导致未经羟自由基处理的脱细胞-核DNA模型的DNA断裂。结论甲醛和乙醛均可直接与脱细胞-核DNA断片形成加合物和/或产生DNA-DNA交联,且形成加合物和/或DNA-DNA交联的能力随醛类烷基数量的增加而减弱。
Objective To study the effects of formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, hexanal and pentanal on acellular-nuclear DNA. Methods Formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, hexanal and pentanal were respectively prepared into 10 mmol / L solution as test substance by using buffer. The acellular-nuclear DNA fragment model of hydroxyl radical damage The ability of the test substance to interact with the DNA; and the degree of DNA fragmentation that results from the test substance being detected using a deoxycytidine-free decellularization-nuclear DNA model. The two models were all six parallel plates with a processing time of 60 min. DNA tailing was detected by comet assay. Fifty decellularized nuclear DNA samples were analyzed by comet assay software (Comet A1.0) The differences between groups were statistically analyzed by SPSS 18.0 software. RESULTS: Formaldehyde-treated acellular-nuclear DNA fragmentation model did not swim out of the DNA fragments. The amount of DNA fragments eluted in the acetaldehyde-treated acellular-nuclear DNA fragment model was significantly lower than that in the control group. There was no significant difference in the amount of DNA fragments electrophoresed from the DNA fragment model compared with the control group; all of the aldehydes did not result in DNA cleavage of the acellular-nuclear DNA model without hydroxyl radical treatment. Conclusions Both formaldehyde and acetaldehyde can directly form adducts and / or produce DNA-DNA cross-links with decellularized-nuclear DNA fragments and the ability to form adducts and / or DNA-DNA cross-links increases with the number of aldehyde alkyl groups Weakened.