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卵巢癌的某些组织病理学变化可作为其生存率的预测因素。最近,采用新方法如 DNA 细胞计数和形态测定法结合传统临床病理特征,对 MountVernon 医院1956年~1978年收治的91例Ⅰ~Ⅵ期卵巢癌进行预后分析。DNA 细胞计数法:福尔根染色涂片法显示 DNA,结合显微光密度计的 Deeley法作 DNA 细胞计数。22例以染色体计数单独计算 DNA 指数。用机械方法将组织制成悬浮液,经低渗盐水和秋水仙碱处理,用醋酸地依红或吉姆萨染色。一部分标本显示 G 带或 C 带,一部分不显带。组织学方法:用有丝分裂活动指数(MAI)
Some histopathological changes in ovarian cancer can be used as predictors of their survival. Recently, 91 patients with stage I-VI ovarian cancer admitted to Mount Vernon Hospital from 1956 to 1978 were retrospectively analyzed using new methods such as DNA cytometry and morphometry combined with traditional clinicopathological features. DNA Cell Counting Method: Forzingen stained smears show DNA, combined with Deeley’s densitometry for DNA cell counting. 22 cases of chromosomal count alone calculated DNA index. Tissue is made into a suspension by mechanical means, treated with hypotonic saline and colchicine, and reddened with acetic acid or by Giemsa. Some specimens showed G band or C band, some not banding. Histological methods: using mitotic activity index (MAI)