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利用自主性Mutator转座子玉米材料115F,330I,715D与玉米自交系材料B73,Mo17,97108,H9-21杂交,获得F1插入诱变群体,F1自交得F2群体.在田间观察F2单株的生物学特征及农艺学性状的表型变异,得到不同表型的突变体,对突变体进行了统计分析.在干旱胁迫下,利用远红外热成像仪对室内小钵种植的F2群体三叶期幼苗进行叶片温度的大规模扫描检测,选取叶温有明显差异的植株作为潜在突变株,共检测了38000余株,成功筛选到108株抗旱单株,121株旱敏感单株.通过重复红外温度检测、叶绿素荧光分析、叶片失水、光合特性分析和可溶性糖类、可溶性蛋白和脯氨酸含量测定对突变体进行了初步的鉴定分析.利用MuTAIL-PCR对获得的突变体进行基因克隆.该研究为深入开展玉米抗旱育种提供了实验材料.
F2 mutants were obtained by crossing mutants transposon 115F, 330I, 715D and maize inbred lines B73, Mo17, 97108 and H9-21, and F1 selfing to F2 population.F2 The biological characteristics and the phenotypic variation of agronomic traits were obtained, and the mutants with different phenotypes were obtained and the mutants were analyzed statistically.Under the drought stress, the foxtail millet Leaf temperature of the leaf stage of the large-scale scanning detection, select the leaf temperature significantly different plants as potential mutants, a total of more than 38000 strains were detected, 108 strains of drought-resistant plants were successfully screened, 121 strains of drought-sensitive plants by repeated The mutant was identified by infrared temperature detection, chlorophyll fluorescence analysis, leaf water loss, photosynthetic characteristics, soluble carbohydrate, soluble protein and proline content.The mutants were cloned by MuTAIL-PCR The research provided experimental materials for further research on drought resistance breeding of maize.