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蛋白质组学研究和药物开发迫切需要高通量、高精度、无标记且实时检测技术。该文提出了一种基于干涉成像的表面等离子共振(SPR)生物分子相互作用检测方法,在入射光的p和s偏振分量之间分别附加90°和-90°位相差,采集对应的干涉图像,并利用所采集的2帧图像,计算出反映传感面折射率分布的信号。循环采集能够实时获得生物反应进行时传感表面发生的折射率分布变化。这种方法在克服光源非均匀性影响的同时,还抑制了光强漂移引起的测量误差,达到了高精度。实验结果表明:干涉成像的图像一致性好,系统的折射率分辨率达到了2×10-6RIU(refractive index unit),动态检测区间大于0.005 RIU。在此基础上,检测了兔IgG和羊抗兔IgG的相互作用,结果表明表面等离子体共振干涉成像系统适于高通量检测,完善后有可能成为一种蛋白质组学研究和药物开发的强有力工具。
Proteomics research and drug development urgently require high-throughput, high-precision, label-free and real-time detection techniques. In this paper, a method of surface plasmon resonance (SPR) biomolecular interaction detection based on interference imaging is proposed. The phase difference between the p and s polarization components of incident light is added by 90 ° and -90 ° respectively, and the corresponding interference image is acquired , And use the collected 2 frames of image to calculate the signal reflecting the refractive index distribution of the sensing surface. Loop acquisition enables real-time changes in the refractive index profile of the sensing surface as the biological response proceeds. This method overcomes the influence of non-uniformity of light source, but also restrain the measurement error caused by light intensity drift to achieve high precision. The experimental results show that the interference image has good image consistency, the refractive index resolution of the system reaches 2 × 10-6 RIU (refractive index unit), and the dynamic detection interval is greater than 0.005 RIU. On this basis, the interaction between rabbit IgG and goat anti-rabbit IgG was detected. The results show that surface plasmon resonance interferometry imaging system is suitable for high-throughput detection, which may become a strong proteomics research and drug development Powerful tool.