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目的 :探讨将RhoA/Rho激酶抑制剂Y 2 76 32涂抹于阴茎白膜表面以及阴茎龟头皮肤表面有无促进阴茎勃起的作用及其对体循环的影响。 方法 :2 0只成年雄性SD大鼠 ,体重为 2 5 0～ 30 0g ,随机分为实验组和对照组。全身麻醉后颈动脉和海绵体插管连续监测平均动脉压 (MAP)和阴茎海绵体内压 (CCP)的变化。寻找盆腔星状神经节(MPG)并以系列电刺激诱发勃起。向海绵体白膜表面及阴茎龟头皮肤表面涂抹Y 2 76 32 ,观察用药前后阴茎勃起的改变及MAP的改变。 结果 :将Rho激酶抑制剂Y 2 76 32涂抹于大鼠阴茎白膜表面和阴茎龟头皮肤表面均可促进在无阴茎支配神经电刺激下发生勃起 (ICP/MAP明显升高 ) ,在系列电刺激MPG时用药后发生的勃起反应较用药前亦明显增强。表面用药后还发现大鼠体循环血压有不同程度的降低。 结论 :阴茎皮肤表面应用Rho激酶抑制剂是一种安全有效的ED治疗方法 ,其临床应用价值仍有待于进一步探索
Objective: To investigate the effect of RhoA / Rho kinase inhibitor Y 2 76 32 applied to the surface of penile albuginea and penis glans penis to promote penile erection and its effect on the systemic circulation. Methods: Twenty adult male Sprague-Dawley rats weighing 250 to 300 g were randomly divided into experimental group and control group. The changes of mean arterial pressure (MAP) and penis pressure (CCP) were continuously monitored by carotid and cavernous cannula after general anesthesia. Look for pelvic stellate ganglion (MPG) and induce erection with a series of electrical stimuli. To the sponge white film surface and penis glans skin surface smear Y27632, observed before and after treatment of penile erection changes and MAP changes. Results: The application of Rho kinase inhibitor Y 2 76 32 to the surface of penile white albuginea and penis glans penis could promote the erection without ICP stimulation (ICP / MAP). In the series of electrical stimulation MPG erectile reaction occurred after medication than before treatment also significantly increased. Surface medication also found that there is varying degrees of systemic blood pressure in rats decreased. Conclusion: The application of Rho kinase inhibitor on the skin surface of penis is a safe and effective ED treatment, and its clinical application remains to be further explored