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本文报道了以~3H-葡萄糖胺的掺入判断PGE_2对滤泡壁新合成蛋白多糖的影响。从22名绝育做小剖腹术妇女中得到不同时期的滤泡和黄体,每个卵巢切一小块做活检并转移到冰冷的Krebs-Ringer 重碳酸钠缓冲液(KRB)中,分离滤泡并穿刺,使其释放颗粒细胞,用Dennefors(1982)提出的参数进行分类,其中11个滤泡属排卵前,<48°,其余为未成熟的,但不是闭锁滤泡(排卵前2～6天)。将白膜从膜层剥下切成片(湿重约2 mg),先在通
In this paper, the effect of PGE 2 on the newly synthesized proteoglycan in the follicular wall was evaluated by incorporation of ~ 3H-glucosamine. Follicles and corpus luteum were obtained from 22 small, sterile laparotomy women at various stages. Each ovary was sectioned with a small biopsy and transferred to ice-cold Krebs-Ringer bicarbonate buffer (KRB) and the follicles were separated Piercing to release granulosa cells was performed using the parameters set forth by Dennefors (1982), with 11 follicles <48 ° prior to ovulation and the remaining immature but not blocked follicles (2 to 6 days before ovulation) ). The white film peeled off from the film cut into pieces (wet weight about 2 mg), first in the pass