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本实验用缺乏维生素D的饲料饲喂刚断奶的SD大鼠,观察这种饲料对血清钙水平的影响,对照大鼠的饲料除了适当量的维生素D外,其它成分都相同。维生素D缺乏大鼠的血浆钙浓度同时对照大鼠一样,而且试验组的生长速度至少在6个月内是正常的。维生素D缺乏可用以下几种方法证明:在6周后检测不出血浆1,25-二羟维生素D_3(1,25-二羟胆钙化甾醇)水平;4个月后骺生长板增大;在应力试验下股骨变弱;在分离的肾细胞中25-羟基维生素D1-羟化酶活性增加。在平行试验中,给哺乳雌大鼠喂同样的维生素D缺乏饲料。幼鼠的血浆钙水平减少并维持低水平。在这些幼鼠中也存在阻碍生长的情况。这可能表明在哺乳期母鼠的维生素D恶化会引起在后代调控体内钙稳定过程中的持
In this experiment, weaned SD rats were fed a diet deficient in vitamin D to observe the effect of this diet on serum calcium levels. The control rats had the same diet with the same amount of vitamin D as the control. The plasma calcium concentration of vitamin D deficient rats was the same as that of the control rats, and the growth rate of the test group was normal for at least 6 months. Vitamin D deficiency can be proven by the following methods: No plasma 1,25-dihydroxyvitamin D3 (1,25-dihydroxycholecalciferol) levels were detected after 6 weeks; the epiphyseal growth plate increased after 4 months; at The femur became weaker under stress test; 25-hydroxyvitamin D1-hydroxylase activity increased in isolated kidney cells. In parallel experiments, lactating female rats were fed the same vitamin D deficient diet. Young rats have reduced and maintained low plasma calcium levels. There is also a hindrance to growth in these young rats. This may indicate that worsening vitamin D in lactating maternal mothers may result in maintenance of calcium in the offspring in vivo