Cloning and expression and immunogenicity of Helicobacter pylori BabA2 gene

• 作 者: Yang Bai Ya-Li Zhang Ye Chen Jian-Feng Jin Zhao-Shan Zhang Dian-Yuan Zhou
• 机 构: PLA Institute for Digestive Medicine, Nanfang Hospital, the First Military Medical University, Guang
• 出 处: 世界胃肠病学杂志（英文版）
• 发表日期: 2004年17期
• 关键词: recombinant protein Helicobacter pylori sequence analysis expression vector sequ

AIM: To construct a recombinant strain which expresses BabA of Helicobacter pylori(H pylori) and to study the immunogenicity of BabA.METHODS: BabA2 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+) and expressed in the BL21 (DE3) E. coli strain. Furthermore,BabA immunogenicity was studied by animal test.RESULTS: DNA sequence analysis showed the sequence of BabA2 DNA was the same as the one published by GenBank.The BabA recombinant protein accounted for 34.8% of the total bacterial protein. The serum from H pylori infected patients and Balb/c miced immunized with BabA itself could recognize rBabA.CONCLUSION: BabA recombinant protein may be an potential vaccine for control and treatment of H pylori infection.