雷公藤多甙对大鼠急性肝衰竭的保护作用

• 作 者: 张兴国 吕敏和 姜会敏 马立宪 吕涛 贾一韬 许复郁
• 机 构: 山东医科大学,山东医科大学卫生化学教研室,山东省立医院职业病科
• 出 处: 新消化病学杂志
• 发表日期: 1997年12期
• 关键词: 急性肝衰竭 雷公藤多甙 大鼠 肝功能衰竭 治疗应用 肝组织病理 中医药疗法 疾病模型 生理盐水溶液 染色质凝聚

目的探讨雷公藤多甙（ＧＴＷ）对实验性肝衰竭的保护作用．方法雄性Ｗｉｓｔａｒ大鼠２４只，随机分为对照组（ｎ＝８），急性肝衰竭（ＡＨＦ）组（ｎ＝８）和ＧＴＷ保护组（ｎ＝８）．ＧＴＷ组在实验前５ｄＧＴＷ２５ｍｇ／（ｋｇ·ｄ）经胃管灌胃，其余两组均以等量生理盐水溶液灌胃．从第６天开始，ＡＨＦ组和ＧＴＷ组均在空腹１２ｈ后ｉｐＤＧａｌＮ１６ｇ／ｋｇ，注射后４０ｈ，以３０ｇ／Ｌ戊巴比妥钠（４０ｍｇ／ｋｇ）ｉｐ麻醉，心脏抽血测定血清ＡＬＴ，ＴＢ和Ｔ淋巴细胞亚群．同时，在光镜和电镜下观察各组肝组织病理变化．结果ＡＨＦ组ＡＬＴ（ＩＵ／Ｌ）和ＴＢ（μｍｏｌ／Ｌ）分别为７８２８±５７６２和１２５６２７±６７０２７；ＯＸ８水平为１４０％±３％．ＧＴＷ组ＡＬＴ和ＴＢ分别为３５９±５４和４７±３５；ＯＸ８为４３％±４％．两组比较有显著统计学差异（Ｐ＜００５或００１）．同时，前者电镜下线粒体和内质网肿胀、破损，核内染色质凝聚，后者细胞器受损明显减轻．结论雷公藤多甙对实验性急性肝衰竭具有保护作用．

Objective To investigate the protective effects of tripterygium glycosides (GTW) on experimental liver failure. Methods Twenty - four male Wistar rats were randomly divided into control group (n = 8), acute liver failure group (n = 8) and GTW protection group (n = 8). The rats in GTW group were given gTW25mg / (kg · d) by gavage before the experiment, and the other two groups were given gavage with saline. From the 6th day, AHF group and GTW group were ipD GalN1.6g / kg 12h after fasting, anesthetized with 30g / L sodium pentobarbital (40mg / kg) ip 40h after injection, ALT, TB and T lymphocyte subsets. At the same time, pathological changes of liver in each group were observed under light microscope and electron microscope. Results The ALT (IU / L) and TB (μmol / L) in AHF group were 7828 ± 5762 and 125627 ± 67027, respectively. The OX8 level was 140% ± 3%. ALT and TB in the GTW group were 359 ± 54 and 47 ± 35, respectively; OX8 was 43% ± 4%. There was significant difference between the two groups (P <005 or 001). At the same time, the former under electron microscope, mitochondria and endoplasmic reticulum swelling, damage, condensation of nuclear chromatin, which was significantly reduced organelle damage. Conclusion Tripterygium glycosides have a protective effect on experimental acute liver failure.