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目的研究法尼酯X受体(farnesoid X receptor,FXR)对肝型脂肪酸结合蛋白(liver fatty acid-binding pro-tein,L-FABP)表达的影响。方法用终浓度为50、100μmol/L的鹅脱氧胆酸(chenodesoxycholic acid,CDCA)以及1、5μmol/L的GW4064分别处理人胚胎肝细胞LO2和人肝癌细胞株HepG2后,用逆转录-聚合酶链反应(RT-PCR)法检测FXR特异靶基因小异二聚体配体(small heterodimer partner,SHP)和L-FABP mRNA表达,再用Western blot法检测L-FABP蛋白水平的变化。结果LO2和HepG2细胞经FXR特异性激动剂CDCA以及GW4064刺激后,细胞内的SHP mRNA水平均明显升高,表明FXR在这两种细胞中是有功能活性的;而L-FABP mRNA和蛋白水平显著下降(P<0.05)。结论激活后的FXR可抑制L-FABP的表达活性。
Objective To investigate the effect of farnesoid X receptor (FXR) on the expression of liver fatty acid-binding pro-tein (L-FABP). Methods Human embryonic hepatocytes LO2 and human hepatocellular carcinoma cell line HepG2 were treated with chenodesoxycholic acid (CDCA) at final concentrations of 50 and 100 μmol / L and GW4064 at 1 and 5 μmol / L, respectively. The mRNA expression of small heterodimer partner (L-FABP) and FXR specific target gene were detected by RT-PCR. The protein level of L-FABP was detected by Western blot. Results The levels of SHP mRNA in LO2 and HepG2 cells stimulated by FXR-specific agonists CDCA and GW4064 were significantly increased, indicating that FXR was functionally active in both of these cells, whereas L-FABP mRNA and protein levels Significantly decreased (P <0.05). Conclusion The activated FXR can inhibit the expression of L-FABP.