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不断新生的各层鳞状上皮成为检查细胞形成、成熟和死亡调节机制的一种重要的模型.本研究利用剔除了真皮成分的各层表皮分别加入肾上腺素能受体兴奋剂及环磷酸腺甙(cAMP)衍生物,进行体外试验,视其糖酵解和结合氨基酸的速率来检查它们对于正常表皮与增生表皮代谢率的影响.试验结果表明,α-和β-受体混合兴奋剂肾上腺素和β-受体兴奋剂异丙肾上腺素在其剂量超过1×10-~9至1×10-~5M浓度范围时,能抑制正常表皮的糖酵解和氨基酸的结合率.外源性的cAMP衍生物双丁酰cAMP能使表皮代谢活性降低73～78%.当加入茶硷后,该抑制作用尚可随之增强,α-受体兴奋剂去甲肾上腺素亦可引起类似的活性降低,但若用正十六烷处理标本,促其发生轻度增生,
The constantly emerging layers of squamous epithelium become an important model to check the regulatory mechanism of cell formation, maturation and death.In this study, the exclusion of adrenergic receptor agonists and cyclic adenosine monophosphate (cAMP) derivatives were tested in vitro and examined for their effects on normal epidermis and hyperplasia epidermis metabolism, depending on the rate of glycolytic and amino acid binding.The results show that the alpha- and beta-receptor agonist epinephrine And β-receptor agonist isoproterenol inhibited normal epidermal glycolysis and amino acid binding at doses exceeding 1 × 10- ~ 9 to 1 × 10- ~ 5 M. Exogenous The cAMP derivative bisbutyryl-cAMP reduced the epidermal metabolic activity by 73-78%, and the inhibitory effect was enhanced when theophylline was added, and the similar activity was also induced by the alpha-receptor agonist norepinephrine , But if n-hexadecane treatment of specimens to promote its occurrence of mild hyperplasia,