以胞质分裂阻滞法研究女性年龄与体细胞中21号染色体分离异常的关系

• 作 者: 张坚宣 史庆华 潘淑娟 张锡然 单祥年 余龙
• 机 构: 南京师范大学生物系,南京铁道医学院生物教研室,复旦大学遗传学研究所
• 出 处: 遗传
• 发表日期: 1999年01期
• 关键词: 细胞松弛素B 21号染色体 染色体不分离 年龄效应

２１三体是人类最常见的先天性疾病，约９５％患儿的超数２１号染色体来源于母亲，且随母龄增加患儿出生率也随之增加。本文首次运用胞质分裂阻滞法（ＣＢ法）对不同年龄女性体细胞进行荧光原位杂交，对其２１号染色体的分离情况进行分析。结果表明，随年龄的增加，女性体细胞中２１号染色体不分离也随之增加，但２１号染色体丢失无年龄效应，且２１号染色体不分离频率远高于其丢失。该结果表明，女性体细胞中２１号染色体不分离与生殖细胞同样存在年龄效应

Trisomy is the most common congenital disease in humans. Approximately 95% of children have chromosome 21 from the mother, and their birth rate increases with their mother’s age. In this study, the cytochrome cleavage method (CB method) was used for the first time in situ hybridization of somatic cells of different ages to analyze the chromosome 21 segregation. The results showed that with the increase of age, chromosome 21 in female somatic cells also increased without sequestration, but chromosome 21 was lost without age effect, and the frequency of non-separation of chromosome 21 was much higher than its loss. The results show that female chromosome 21 does not separate from somatic cells in germ cells with the same age effect