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分析非息肉性大肠癌发生的分子机理，为早期诊断和预测预后提供理论依据方法用ＰＣＲ－ＳＳＬＰ方法，引物Ｄ２Ｓ４４１，Ｄ２Ｓ１１９和Ｄ２Ｓ１２３检测３１例大肠癌患者的癌组织和癌旁组织的微卫星ＤＮＡ长度片段多态性，６％聚丙烯酰胺凝胶电泳，硝酸银染色结果３１例患者中，１号病例的癌组织中，三对引物扩增产物均出现微卫星ＤＮＡ长度多态变化。６号病例的癌组织的Ｄ２Ｓ４４１引物扩增产物出现杂合性丢失，Ｄ２Ｓ１１９和Ｄ２Ｓ１２３引物的扩增产物出现微卫星ＤＮＡ长度多态变化，其余２９例患者的癌组织的３对引物扩增产物均未出现异常变化。结论部分散发性非息肉性大肠癌的发生与微卫星ＤＮＡ的不稳定性和杂合性丢失有关。
To analyze the molecular mechanism of non-polyposis colorectal cancer and provide a theoretical basis for early diagnosis and prognosis prediction.Methods PCR-SSLP method, primers D2S441, D2S119 and D2S123 were used to detect the microsatellite DNA in cancer tissue and paracancerous tissue in 31 patients with colorectal cancer. Length fragment polymorphism, 6% polyacrylamide gel electrophoresis, silver nitrate staining results 31 cases, the cancer tissue in the 1 case, three pairs of primers amplified products showed polymorphic changes in the length of microsatellite DNA. The amplification products of D2S441 primers in cancer tissue from case 6 showed loss of heterozygosity. The amplification products of D2S119 and D2S123 primers showed polymorphic changes in the length of the microsatellite DNA. The amplification products of the three pairs of primers in the remaining 29 cases were all amplified products. No abnormal changes have occurred. Conclusion The occurrence of some sporadic nonpolyposis colorectal cancers is related to the loss of microsatellite DNA instability and heterozygosity.