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土壤盐碱化已成为危害植物生长的重要因素之一。本研究中,我们对盐碱胁迫下两种桃树(GF677和毛桃)的根部分别进行细胞学观察和比较转录组分析。扫描电子显微镜结果表明GF677根部的根毛密度要显著多于毛桃根部。比较转录组分析显示在|log2 Ratio|≥1和Probability>0.8的阈值下共检测到1 379个基因差异表达,其中279个上调,1 100个下调。对差异表达基因进行Gene ontology(GO)聚类共得到27个小类显著富集。Pathway富集分析显示脯氨酸代谢、类黄酮代谢和氧化磷酸化代谢等通路在GF677中被显著激活,暗示了GF677可能通过提高渗透压和维持氧化还原平衡使植物体免受盐碱胁迫的危害。研究结果为耐盐碱基因挖掘以及桃树耐盐碱分子改良育种提供了理论依据。
Soil salinization has become one of the important factors that endanger plant growth. In this study, we observed the cytology and compared the transcriptome analysis of the roots of two peach trees (GF677 and peach) under salt-alkali stress. Scanning electron microscopy results showed that root density of GF677 root was significantly higher than that of peach root. A comparative transcriptome analysis showed that a total of 1 379 genes were differentially expressed under the log2 Ratio | ≥1 and Probability> 0.8 thresholds, with 279 up-regulated and 1,100 down-regulated. A total of 27 subtypes were significantly enriched by Gene ontology (GO) clustering of differentially expressed genes. Pathway enrichment analysis showed that pathways such as proline metabolism, flavonoid metabolism and oxidative phosphorylation metabolism were significantly activated in GF677, suggesting that GF677 may be harmed by salt stress by increasing osmotic pressure and maintaining a redox balance . The results provide a theoretical basis for salt-resistant gene mining and improvement of salt-and-alkali-resistant molecular breeding in peaches.