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在产前诊断细胞遗传学异常时,嵌合型仍然是一个重要的问题。当在羊水细胞培养基内发现两种以上的细胞株时,有两种因素使得不能作出正确诊断。首先,体外培养的羊水细胞中细胞遗传学异常可能偏高,而不能准确地反映胎儿的染色体结构成分。其次,在羊水细胞培养物中发现的异常细胞的比例可能与体内异常细胞的实际比例有较大的差异。诊断嵌合型的可靠方法是分析每份羊水样本所建立地几份单独培养物的细胞,当在二份以上的培养物内识别出相同的染色体异常时,可以准确地判断体内存在的异常,但
In prenatal diagnosis of cytogenetic abnormalities, chimerism remains an important issue. When more than two cell lines are found in amniotic fluid cell culture, two factors make it impossible to make a correct diagnosis. First, cytogenetic abnormalities in amniotic fluid cells cultured in vitro may be high, but can not accurately reflect the fetal chromosomal structural components. Second, the proportion of abnormal cells found in amniotic fluid cultures may differ significantly from the actual proportion of abnormal cells in the body. A reliable method of diagnosing chimerism is to analyze cells of several individual cultures in each of the amniotic fluid samples and to determine exactly the presence of abnormalities in the body when identical chromosomal abnormalities are identified in more than two cultures, but