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迄今恶性疟原虫体外培养法至少需每天更换培养液一次,浪费人力和物力。作者试图对此作出改进。实验系用恶性疟原虫体外抗氯喹的SGE-1/塞内加尔株,先按Trager-Jensen(1976)法进行体外培养后(培养基谷氨酰胺含量增加到5mM),以1,000转/分离心收集原虫,配成50%的红细胞悬液,再加入正常人A型红细胞使原虫感染率稀释到0.5%或以下。一组仍按上法培养(RPMI组),另一组培养基中添加50mg/l次黄嘌呤(RPMI/HYPOX组)。培养时两组培养皿内皆加入
So far, Plasmodium falciparum in vitro culture method at least once a day to replace the culture medium, a waste of manpower and material resources. The author tries to make improvements to this. In vitro, the SGE-1 / Senegal strain against Plasmodium falciparum resistant to chloroquine was cultured in vitro (the glutamine content of the medium was increased to 5 mM) according to the method of Trager-Jensen (1976) and the protozoal was collected by centrifugation at 1,000 rpm , Dubbed 50% of the red blood cell suspension, then add normal type A red blood cells to protozoal infection rate diluted to 0.5% or less. One group was still cultured according to the above protocol (RPMI group) and the other group was supplemented with 50 mg / l hypoxanthine (RPMI / HYPOX group). Cultured two sets of Petri dishes are added