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目的探讨环氧化酶-1(COX-1)在肾癌中表达情况及判断预后的价值。方法应用实时荧光定量PCR(RT-qPCR)法检测COX-1在42例配对肾癌及癌旁正常组织中mRNA水平,应用免疫组织化学染色的方法检测COX-1在196例肾癌组织(包括91例配对癌旁组织)中蛋白表达水平,统计学方法分析COX-1表达水平与肾癌患者临床病理特征及预后之间的关系。结果 RT-qPCR检测结果显示肾癌组织中COX-1mRNA水平明显高于癌旁正常组织(P<0.001)。免疫组织化学染色及统计分析结果显示COX-1在肾癌中表达上调(P<0.001),196例肾癌标本中有131例(66.8%)COX-1高表达,其高表达与肿瘤大小(P=0.002)、临床分期(P=0.003)、TNM分期(P分别为0.003、0.007、0.027)及肿瘤复发(P<0.001)有关。生存分析结果显示COX-1高表达者预期生存时间较短(P<0.001)。结论本研究发现COX-1在肾癌中高表达,COX-1可能成为一个较理想的肾癌预后标志物。
Objective To investigate the expression of cyclooxygenase-1 (COX-1) in renal cell carcinoma and its prognostic value. Methods The mRNA expression of COX-1 in 42 matched renal cell carcinoma tissues and adjacent normal tissues was detected by real-time fluorescence quantitative PCR (RT-qPCR). Immunohistochemical staining was used to detect the expression of COX-1 in 196 renal carcinoma tissues 91 paired paracancer tissues). The relationship between the expression of COX-1 and clinicopathological features and prognosis of renal cell carcinoma was analyzed statistically. Results The results of RT-qPCR showed that the level of COX-1 mRNA in renal cell carcinoma tissues was significantly higher than that in adjacent normal tissues (P <0.001). Immunohistochemical staining and statistical analysis showed that COX-1 was upregulated in renal cell carcinoma (P <0.001), 131 cases (66.8%) of 196 cases of renal cell carcinoma were highly expressed COX-1, which was correlated with tumor size P = 0.002), clinical stage (P = 0.003), TNM stage (P = 0.003,0.007,0.027, respectively) and tumor recurrence (P <0.001). Survival analysis showed that patients with high COX-1 expression had a shorter survival time (P <0.001). Conclusions This study found that COX-1 is overexpressed in renal cell carcinoma and COX-1 may be a better prognostic marker of renal cell carcinoma.