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目的:研究长梗南五味子抗乙型肝炎病毒(HBV)活性及对急性肝损伤的保护作用。方法:体外实验采用Hep G2.2.15细胞为模型,检测长梗南五味子药物血清对细胞培养上清中乙型肝炎表面抗原(HBs Ag)和乙型肝炎e抗原(HBe Ag)的抑制作用;体内实验采用D-氨基半乳糖(D-Gal N)致小鼠急性肝损伤模型,将健康小鼠随机分为正常对照组、模型组、联苯双酯组(200 mg/kg)、长梗南五味子高、中、低剂量组(生药102.6、51.3、25.65g/Kg),每组10只,每天灌胃1次,连续10天,检测各组动物血清中丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)的活性,HE染色观察肝组织病理学改变。结果:体外实验:长梗南五味子药物血清高、中、低剂量组Hep G2.2.15细胞分泌HBs Ag和HBe Ag水平明显低于空白对照组(P<0.01);体内实验:长梗南五味子高剂量组血清ALT、AST含量分别为(21.88±9.96)、(39.72±7.18)IU/L,与模型组比较显著降低(P<0.01),小鼠肝脏的坏死和炎症病变也有一定改善。结论:长梗南五味子具有显著的抗HBV作用,对小鼠急性肝损伤有明显的保护作用。
Objective: To study the anti-hepatitis B virus (HBV) activity of long-stem-dried schisandra chinensis and its protective effect on acute liver injury. Methods: Hep G2.2.15 cells were used as a model in vitro to test the inhibitory effect of long-tailed schisandra chinensis serum on the inhibition of hepatitis B surface antigen (HBs Ag) and hepatitis B e antigen (HBe Ag) in the cell culture supernatant. The model of acute liver injury induced by D-galactosamine (D-Gal N) was used in the experiment. The healthy mice were randomly divided into normal control group, model group, bifendate group (200 mg / kg) Schisandra high, middle and low dose group (crude drug 102.6,51.3,25.65g / Kg), each group of 10, gavage once a day for 10 consecutive days, serum alanine aminotransferase (ALT) And aspartate aminotransferase (AST) activity, liver histopathological changes were observed. Results: In vitro, the levels of HBs Ag and HBe Ag secreted by Hep G2.2.15 cells in serum of high, medium and low dose groups were significantly lower than those in the blank control group (P <0.01). In vivo experiments: The levels of serum ALT and AST in the dose group were (21.88 ± 9.96) and (39.72 ± 7.18) IU / L respectively, which were significantly lower than those in the model group (P <0.01). The necrosis and inflammation of the liver were also improved. Conclusion: Longgannan Schisandra has a significant anti-HBV effect and has a significant protective effect on acute liver injury in mice.