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Plants adapt to phosphorus (P) deficiency through a complex of biological processes and many genes are involved.Tomato (Solanum lycopersicum L.'Hezuo906’) plants were selected to grown hydroponically to study the temporal and spatial gene expression patterns of the 14-3-3 gene family and their roles in response to P deficiency in tomato plants.Using real-time reverse-transcriptase polymerase chain reaction (RT-PCR),we investigated the expression profiles in different tissues (root,stem and leaf) at short-term and long-term P-deficient stress phases.Results revealed that i) four members of 14-3-3 gene family (TFT1,TFT4,TFT6 and TFT7)were involved in the adaptation of tomato plants to P deficiency,ii) TFT7 responded quickly to P deficiency in the root,while TFT6 responded slowly to P deficiency in the leaf,iii) expression response of TFT4 to P-deficient stress was widely distributed in different tissues (root,stem and leaf) while TFT8 only displayed stem-specific expression,and iv) temporal and tissues-specific expression patterns to P deficiency suggested that isoform specificity existed in tomato 14-3-3 gene family.We propose that TFT7 (one member of ε-like group in tomato 14-3-3 family) is the early responsive gene and may play a role in the adaptation of tomato plants to short-term P deficiency,while TFT6 (one member of non-ε group in tomato 14-3-3 family) is the later responsive gene and may play a role in the adaptation of tomato plants to long-term P deficiency.