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A monolithic polymer column with mixed-mode function was prepared by in situ polymerization in a 1000μL pipette.Two kinds of monomers,butyl methacrylate (BMA) and 2-(dimethylamino)ethyl methacrylate (DMAM),were applied to constructing the mixed-mode function for the synthesized polymer monolith.Its solid-phase extraction properties for liquiritigenin (LQG) were evaluated by high performance liquid chromatography (HPLC) with a gradient elution procedure.The extraction procedure was optimized in experiments.And then,the maximum binding capacity and extraction recovery following the optimal extraction procedure were also investigated.Calibration curve was expressed as A=65.9C+4.53 (r2 =0.998) with a linear range of 0.151～1.80μg/ml.The limit of quantification (LOQ) was estimated as 0.015μg/ml and the limit of detection (LOD) as 0.0045μg/ml.The experimental results indicate that the monolithic polymer pipette presents good extraction efficiency for LQG.The monolithic polymer envisages the potential for application to the enrichment of other flavonoids compounds being similar to the structure of LQG.