论文部分内容阅读
目的对河北省2012年-2013年流行性脑脊髓膜炎(流脑)病例病原学监测结果进行分析,了解脑膜炎奈瑟菌(Neisseria meningitidis,Nm)的菌群种类及分子分型特征。方法对2012年-2013年从流脑病例中分离的脑膜炎奈瑟菌菌株进行培养、生化鉴定、血清学鉴定、种属及分群的荧光定量PCR检测以及多位点序列分型(MLST)和por A基因分型研究;并对2012年-2013年流脑病例的脑脊液和血清标本进行种属、分群的荧光定量PCR检测及MLST分析。结果2012年-2013年,从流脑病例中共检测出B群5例,C群14例,W135群2例;MLST结果显示除1例没有所属的克隆群外,其余16例标本均属于ST-4821克隆群;6株Nm菌株的por A基因分属于不同的型别。结论河北省2012年-2013年流脑病例以C群为主,B群流脑呈现增长趋势,本省首次出现了W135群流脑病例,病例大都为高致病性ST-4821克隆群,提示应加强本省Nm的监测。
Objective To analyze the etiological surveillance results of epidemic cerebrospinal meningitis (EC) cases from 2012 to 2013 in Hebei Province, and to understand the species and molecular typing characteristics of Neisseria meningitidis (Nm). Methods The strains of Neisseria meningitides isolated from patients with meningitis during 2012-2013 were cultured, biochemically identified, serologically characterized, and the species and population were analyzed by real-time quantitative PCR and MLST. por A genotyping studies. Species and cluster PCR and MLST analysis of cerebrospinal fluid (CSF) and serum samples of patients with cerebellar encephalitis from 2012 to 2013 were performed. Results From 2012 to 2013, there were 5 cases in group B, 14 cases in group C and 2 cases in group W135. The results of MLST showed that the other 16 cases belong to ST- 4821 clones. The por A genes of 6 Nm strains belong to different genotypes. Conclusion The epidemic encephalitis in Hebei Province from 2012 to 2013 was mainly in group C, and the meningitis in group B showed an increasing trend. For the first time, there were cases of W135 group meningoencephala in Hebei Province, most of which were highly pathogenic ST-4821 clones, suggesting that Strengthen the monitoring of the province’s Nm.