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以甘蓝型油菜的28个基因组探针和两种限制性内切酶对包括46个中国品种、9个欧洲品种在内的59个甘蓝型油菜品种(系)的RFLP标记进行了分析。在放射自显影胶片上,共检测到410条具多态性的分子杂交带,表明甘蓝型油菜中存在着极为丰富的遗传变异。聚类分析结果表明,在相似性为45%的水平上,可把中国甘蓝型油菜划分为6组:胜利油菜组、跃进油菜组、中油821组、远缘种质组、优质油菜组和变异不详组。欧洲冬油菜与以上6组存在着较显著的遗传距离。主成分分析的结果与上述分组较为一致。以上结果表明,对于扩大中国甘蓝型油菜的遗传基础,欧洲冬油菜无疑是一个重要的种质资源。另一方面,用典型的中国甘蓝型油菜与欧洲冬油菜配制的杂交种,较易产生强大的杂种优势。从对已进行了染色体定位的61条放射自显影带的分析看,无论是上述分组内,还是分组间,RFLP的相对差异均主要表现在A基因组中。讨论了致使A基因组遗传变异较大的可能因素。
The RFLP markers of 59 Brassica napus cultivars (lines), including 46 Chinese varieties and 9 European varieties, were analyzed with 28 genomic probes and two restriction enzymes of Brassica napus. A total of 410 molecular hybridization bands were detected on autoradiography, indicating that there is a very rich genetic variation in Brassica napus. Cluster analysis showed that the Chinese cabbage rape can be divided into six groups at the similarity level of 45%: victuals of rapeseed group, Yuejin canola group, Zhongyou 821 group, distant germplasm group, high-quality rapeseed group and variation Unknown group. European winter rape and the above six groups there is a more significant genetic distance. The result of principal component analysis is more consistent with the above grouping. The above results show that, for expanding the genetic basis of Chinese Brassica napus, winter rape is undoubtedly an important germplasm resources. On the other hand, it is easier to produce strong heterosis with the hybrids of the typical Chinese Brassica napus and European winter rape. Based on the analysis of 61 autoradiographs that have been chromosomally located, the relative differences of RFLP in both the above groups and among the groups are mainly shown in the A genome. The possible causes for the large genetic variation in the A genome are discussed.