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目的:检测抗VEGFR-2嵌合Fab抗体对裸鼠肝癌原位移植瘤血管生成的影响。方法:建立裸鼠肝癌H22细胞原位移植瘤模型,随机分成生理盐水组(n=12)和抗体组(n=12)。采用免疫组织化学SP染色法对两组肝脏移植瘤进行血管染色,观察其微血管密度(MVD)情况。结果:成功建立裸鼠H22肝癌原位移植瘤模型,HE染色显示肝脏移植瘤为肝细胞肝癌,免疫组化结果显示肝脏实体瘤内微血管密度抗体组较生理盐水组显著性减少(25.64±1.53 vs 8.65±1.79,P<0.05)。结论:抗VEGFR-2嵌合Fab抗体能够抑制裸鼠肝癌原位移植瘤的血管生成。
Objective: To investigate the effect of anti-VEGFR-2 chimeric Fab antibody on angiogenesis in orthotopic transplanted hepatocarcinoma of nude mice. Methods: orthotopic implantation of hepatocellular carcinoma H22 cells in nude mice was established and randomly divided into normal saline group (n = 12) and antibody group (n = 12). Immunohistochemical SP staining was used to stain the blood vessels of two groups of liver xenografts, and the microvessel density (MVD) was observed. Results: The in situ transplanted H22 hepatocarcinoma model was successfully established in nude mice. Hematoxylin and eosin (HE) staining showed that the transplanted hepatocellular carcinoma was hepatocellular carcinoma. The immunohistochemistry results showed that the microvessel density antibody group in liver solid tumor decreased significantly compared with saline group (25.64 ± 1.53 vs 8.65 ± 1.79, P <0.05). Conclusion: The anti-VEGFR-2 chimeric Fab antibody can inhibit angiogenesis in orthotopic transplanted hepatocarcinoma in nude mice.