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L-BC约占慢粒(CML)急变的1/3,且大多数显示B-细胞发育,形态及表型特点与B-谱系急性淋巴细胞白血病(ALL)相同。分析两型肿瘤相关性DNA重组即22号染色体上断裂点簇集区(bcr)和IgH基因重排。可以鉴别Ph′+细胞群和L-BC细胞。尽管这种重排发生在相对狭窄的基因组区,但病人之间每次杂交方式的序列变异常常不同,因此,bcr和IgH异常片段可作为独特的肿瘤标志。作者利用Southern印迹杂交技术。分析了4例发生一次或多次淋巴或混合性BC的CML病人慢性或急性期DNA标本,旨在检测疾病发展中的Ph′+细胞和原始细胞克隆。
L-BC accounts for about 1/3 of CML acute changes, and most of them show B-cell development. Morphology and phenotypic characteristics are the same as B-lineage acute lymphoblastic leukemia (ALL). Analysis of two types of tumor-associated DNA recombination, ie, the cluster site at the breakpoint on chromosome 22 (bcr) and IgH rearrangements. Ph ’+ cell populations and L-BC cells can be identified. Although this rearrangement occurs in a relatively narrow genomic region, the sequence variation in each hybridization pattern among patients often varies, so abnormal fragments of bcr and IgH may serve as unique tumor markers. The authors used Southern blot hybridization. Four chronic or acute DNA specimens of CML patients with one or more episodes of lymphoid or mixed BC were analyzed to detect Ph ’+ cells and primary cell clones in disease development.