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材料与方法材料来源:由湖北省农科院粮作所提供农垦58原始不育株繁殖后代种子。试验方法:材料的种植,1986年4月播种进行初步观察后留蔸越冬,及1987年秧龄30天3/23、4/8、4/15分期播种,单本栽插,每一小区40-60株,种在本所试验田E119°23′,N26°05′,海拔5.4米)。育性检查:采用同野败型不育系镜检方法,从5/11—9/15,共观察18次,检查同株异穗不同时期抽穗的花粉育性,每穗检查5-10朵颖花,用I—IK溶夜染色制片,置于100倍光学显微镜检查花粉粒染色程度,以
Materials and Methods Source of Materials: Progeny from Nongken 58 original sterile plants were sown from the Grain Crops Institute of Hubei Academy of Agricultural Sciences. Test methods: planting materials, planting in April 1986 for the first observation after the stay 蔸 wintering, and 1987 seedling age 30 days 3 / 23,4 / 8,4 / 15 staging seeding, single planting, each district 40- 60 plants, planted in our experimental field E119 ° 23 ’, N26 ° 05’, elevation of 5.4 meters). Fertility Test: The same wild type sterile microscopy microscopy method, from 5 / 11-9 / 15, a total of 18 times to observe the same plant ear at different stages of the heading of the pollen fertility, check 5-10 per ear Spikelet, I-IK lysozyme preparation, placed 100 times optical microscopy pollen grain staining degree to